ABSTRACT
<p><b>OBJECTIVE</b>We have evaluated the direct effect of ampelopsis (APS) on duck hepatitis B virus (DHBV) replication in ducklings in vivo.</p><p><b>METHOD</b>One-day-old ducklings were infected with DHBV. After infection for 7 days, the animals were treated with APS at dosages of 70, 150, 300 mg x kg(-1) of body weight via the oral route. The drug was given twice per day for 10 days continuously, and normal saline was used as control. The blood was drawn from the posterior tibial vein of all ducks before treatment (T0), after the medication for 5 (T5), 10 (T10) days and withdrawal of the drug for 3 days (P3). DHBV DNA in duck serum was detected by dot blot.</p><p><b>RESULT</b>The duck serum DHBV-DNA levels were reduced in the group of APS (150, 300 mg x kg(-1)) after treated for 5 and 10 days and the levels of DHBV-DNA did not markedly relapse in both groups of APS after withdrawal of the drug for 3 days. We provide the first evidence that APS can efficiently inhibits DHBV replication in ducks in vivo.</p><p><b>CONCLUSION</b>APS therefore warrants further investigation as a potential therapeutic agent for HBV infections.</p>
Subject(s)
Animals , Ampelopsis , Chemistry , Antiviral Agents , Pharmacology , DNA, Viral , Metabolism , Drugs, Chinese Herbal , Pharmacology , Ducks , Blood , Virology , Hepatitis B Virus, Duck , Metabolism , Physiology , Virus ReplicationABSTRACT
OBJECTIVE: To discuss the in vitro anti-HBV effect of total flavonoids of Ampelopsis grossedentata from Guangxi. METHODS: The inhibitory effects of total flavonoids of Ampelopsis grossedentata-containing serum on 2215 cells' secretion of HBsAg and HBeAg were determined by ELISA. RESULTS: The secretion of HBsAg and HBeAg in 2215 cells was remarkably inhibited by different concentration of total flavonoids (P
ABSTRACT
OBJECTIVE: To study the activity of flavonoids WF extracted from Mallotus apelta against duck hepatitis B virus (DHBV).METHODS: One-day-old ducklings were infected with DHBV.7 days later the DHBV-infectious model was established successfully.The model ducklings were administrated with WF of 75 and 150 mg?kg-1 or lamivudine of 50 mg?kg-1 b.i.d.via i.g.gtt for 10 days consecutively.Serums were collected at 5th and 10th day after drug administration and 3th days after drug withdrawal.The level of DHBV-DNA in serum was detected by dot-blotting hybridization test.RESULTS: 75 and 150 mg?kg-1 WF reduced the level of DHBV-DNA significantly(P
ABSTRACT
OBJECTIVE:To investigate the inhibitory effect of the total flavoniods of Ampelopsis grossedentata on human hepatoma carcinoma cells(HCC) in vitro.METHODS:The inhibitory effect of the total flavoniods of Ampelopsis grossedentata extract on human hepatoma carcinoma BEL7404 cells in vitro was analyzed by MTT,cell growth curve method and colony formation method,respectively.RESULTS:The total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells in vitro.MTT assay showed that the IC50 was 28.59 ?g?mL-1;the cell growth curve measurement showed that the total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells;the colony formation assay showed that the IC50 was 100% at a drug concentration of above 22.22 ?g?mL-1 and the IC50 was 58.05% at a drug concentration of 14.81 ?g?mL-1.CONCLUSION:The total flavonoids of Ampelopsis grossedentata have a remarkable inhibitory effects on the growth of BEL 7404 in vitro.